Plant Hormonomics: Multiple Phytohormone Profiling by Targeted Metabolomics
نویسندگان
چکیده
منابع مشابه
Integrating metabolomics profiling measurements across multiple biobanks.
To optimize the quality of large scale mass-spectrometry based metabolomics data obtained from semiquantitative profiling measurements, it is important to use a strategy in which dedicated measurement designs are combined with a strict statistical quality control regime. This assures consistently high-quality results across measurements from individual studies, but semiquantitative data have be...
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BACKGROUND Bryophytes represent a very diverse group of non-vascular plants such as mosses, liverworts and hornworts and the oldest extant lineage of land plants. Determination of endogenous phytohormone profiles in bryophytes can provide substantial information about early land plant evolution. In this study, we screened thirty bryophyte species including six liverworts and twenty-four mosses ...
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Methods Homogenization and extraction with organic solvents was done in one microcentrifuge tube and accelerated by crushing the plant material in a vibration mill. The extracts from minute amounts of fresh plant material were immediately purified using a solid-phase extraction (C18, C8, OasisTM HLB cartridges)in combination with ion-exchange and/or immunoaffinity purification. A fast chromatog...
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During the last 15 years, metabolomics has emerged as the latest of the so-called “omics” research fields. It deals with the analytical characterization of the metabolome, i.e. the low molecular weight metabolite complement of the biological system under investigation. A wide range of biological systems have benefited from metabolomics studies, from relatively simple microbes to plants and comp...
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In this work we present a targeted gene expression strategy employing trinucleotide threading (TnT) amplification and massive parallel sequencing. We have previously shown that TnT combined with array readout accurately monitors expression levels. However, with this detection strategy spurious products go undetected. Accordingly, we adapted the TnT protocol to massive parallel sequencing to acq...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 2018
ISSN: 1532-2548
DOI: 10.1104/pp.18.00293